diet for blood type o negative pdf

diet for blood type o negative pdf

diet for blood type o negative pdf

the enzyme-linked immunosorbent assay or elisa is a commonly used format for serologic testing. the purpose of this animation is to explainhow this test works. elisa serologies are usually done in multi-wellmicrotiter plates so that dilutions of serum can be easily prepared and tested. to better understand how this assay is done,lets take a closer look at what happens in

diet for blood type o negative pdf, one of the wells of this assay plate. to perform the assay, the wells of the plate are coatedwith the antigen of interest. for commercial tests, this would be done bythe manufacturer of the assay. to begin testing, the wells are filled withdilutions of the patient's serum.

if antibodies against the antigen are presentthey will bind to the antigen fixed to the bottom of the wells. but only antigen-specific antibodies willbind to the wells. the wells are then washed out to remove allunbound antibodies. next a solution of an animal antibody againsthuman antibodies is added. this second antibody is covalently conjugatedto an enzyme. the wells are washed again, this time to removeany unbound enzyme-conjugated antibody. finally a solution of a colorgenic enzymesubstrate is added. the interaction of the substrate with theenzyme on the second antibody generates visible

color. the development of color in the wells with aspecific antibody can be seen with the naked eye or quantified with an electronic platereader. the reaction is less intense as the serumis diluted and the amount of antibody captured in the wells decreases. the titer is the highest dilution with definitecolor development. subtitles by the amara.org community

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